Profiling of miRNAs and their interfering targets in peripheral blood mononuclear cells from patients with chronic myeloid leukaemia

Introduction MicroRNAs may be implicated in the acquisition of drug resistance chronic myeloid leukemia as they regulate expression not only BCR-ABL1 but also genes associated with activation transfer proteins or essential signaling pathways. Methods To understand impact specifically expressed miRNAs and their target genes, we collected peripheral blood mononuclear cells (PBMC) from patients diagnosed (CML) healthy donors to determine whole miRNA by small RNA sequencing screened out 31 differentially microRNAs (DE-miRNAs) high expression. With utilization set enrichment analysis tools, present here a comprehensive relevance DE-miRNAs disease biological function. Furthermore, literature-based miRNA-target gene database was used analyze overall define responses. We further integrated DE-miRNA identify CML targeted signature singscore (CMTGSS) gene-set (GSEA) correlation between CMTGSS Hallmark gene-sets PBMC samples clinical patients. Finally, association stratification multiple cell lineage sets validated using GSEA. Results Although individual have been reported varying degrees on CML, overall, our results show that abnormally upregulated are apoptosis aberrantly downregulated cycle. The shows defined prognosis CMTGSS-based presented tendency for affect differentiation microenvironment. Conclusion Collectively, this study comprehensively analyzed functions differential genes. specific differentiated immune suggests magnitude potential targets setting. It helps us make links different obtained multi-faceted studies provide more research directions.